WebCyto-ID assay is based on the usage of a specific dye that selectively stains autophagic compartments and therefore allows determination of autophagic flux as accumulation of stained compartments in basic or activated conditions [rapamycin (15 µmol/L-), PP242 (1-5 µmol/L) or Hanks’ Balanced Salt Solution containing 6 mmol/L glucose (starvation … WebCatalog number: S11342. The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find ...

Cell damage evaluation by intelligent imaging flow cytometry

WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature in DMEM without phenol red, containing 5% fetal bovine serum and the fluorescent Cyto-ID probe (Cyto-ID® Autophagy detection kit; Enzo Life Sciences, ENZ-51031K200). WebCyto-ID Green autophagy dye staining procedure for autophagy detection Cyto-ID Green solution was prepared by mixing the dye with 1× assay buffer. The cell sample was … brianefallon twitter

Image Cytometry - an overview ScienceDirect Topics

http://biopioneer.com.tw/?p=41115 WebWe first verified that the Cyto-ID dye specifically labels autophagic compartments with minimal staining of lysosomes and endosomes. We then developed a new Cyto-ID … WebThe cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO … brian edwin