WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release. WebMay 1, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-D-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at very low...
Cytochalasin A and B: INHIBITION OF SUGAR UPTAKE IN …
WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … WebJan 1, 1990 · Cytochalasin B also blocked the uptake of 14 C-L-ascorbic acid (1.25 mg%). The results of these studies further support the hypothesis that glucose and ascorbate … imfdb texas chainsaw massacre
The inhibition of glucose uptake to erythrocytes: microwave
WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the WebIn erythrocytes, adipocytes, and chick embryo fibroblasts, the Ki for glucose uptake inhibition and the Kd for cytochalasin B binding range from 0.1 to 0.4 µM. Values for hepatocytes and liver membranes are an order of magnitude higher … Cytochalasin B inhibits glucose transport [3] and platelet aggregation. It blocks adenosine -induced apoptotic body formation without affecting activation of endogenous ADP -ribosylation in leukemia HL-60 cells. [4] It is also used in cloning through nuclear transfer. See more Cytochalasin B, the name of which comes from the Greek cytos (cell) and chalasis (relaxation), is a cell-permeable mycotoxin. It was found that substoichiometric concentrations of cytochalasin B (CB) strongly inhibit … See more 1960s Cytochalasin B was first described in 1967, when it had been isolated from moulds by Dr W.B. Turner. Smith et al. found that CB … See more Cytochalasin B contains several highly polar keto- and hydroxyl groups and one peripheric lipophilic benzyl unit. See more There are ten possible sites for the in vitro degradation of cytochalasin B. There is not yet any evidence that the same sites are used for degradation in vivo, but evidence has confirmed the in vitro sites. Degradation is initialized by a periodate cleavage of the … See more Cytochalasins can be isolated from the fungi in which they naturally occur. Originally, they were isolated from Helminthosporium dematioideum. Other producers include See more It is suggested that the predominant mechanism of cytochalasin B is the inhibition of actin filament polymerization through binding to … See more Interactions When adding cytochalasin B and the beta-andrenergic agonist (-)-isoproterenol, prostaglandin E1 or cholera toxin to wild type S49 … See more list of pantry items for printing