Determination of aconitase activity

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August undefined, 2024

WebThe aconitase activity was significantly depressed in the 2% O2 group (*P ⬍ 0.03, n ⫽ 6/group). ... Determination of the mechanism of free radical generation in damage in the presence of cyanosis: a new concept of controlled cardiac human aortic endothelial cells exposed to anoxia and reoxygenation. ... WebJun 23, 2024 · Prior studies indicated that H129 is the only histidine ligand involved in the control of the iron donation capability of YtfE, as its replacement for a leucine residue produces a protein unable to restore the aconitase activity . Interestingly, H129 forms an H-bond with E159 (H129 Nδ1 …E159 Oε1 2.66 Å).

Aconitase Activity Assay Kit (MAK051) - Technical …

WebDetermination of Aconitase Activity 4.5 ml. of water in each of a series of thick walled 100 X 14 mm. Pyrex test-tubes are brought to 30”. WebApr 14, 2024 · Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities. The chemical composition of the hydroethanolic extracts, obtained from dried plants of S. incarnata, S. coccinea, and … bit set profi

Aconitase - University of Wisconsin–Madison

WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice … WebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. WebMitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative... Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Springer for Research & Development dataplug by texa was ist das

Prooxidant action of rosmarinic acid: Transition metal …

Bacillus subtilis aconitase is an RNA-binding protein PNAS

WebTotal aconitase activity can also be measured in whole cell lysates, for example, 143B osteosarcoma lysate shown below (Figure 3). Note – the aconitase activity measured in … WebThe osmotic and activity coefficients of aqueous mixtures of NaCl and MgCl/sub 2/ have been determined at 25/sup 0/C by using the isopiestic method. These measurements extend from low concentrations to the crystallization limits of the mixtures. They are critically compared to published isopiestic, direct vapor pressure, and emf data for this system. data plot python 使い方WebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been … data plus online hosting dphsweb.com

"WebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower … " - Determination of aconitase activity

Determination of aconitase activity

(PDF) The Mechanism of Aconitase Action - ResearchGate

WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease January 2024 Methods in molecular biology (Clifton, N.J.)1731:49-56 … WebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953).

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WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease. ... WebThe aconitase activity of a sample may be determined by the following equation: Aconitase Activity = B Sample Dilution Factor (milliunit/mL) T V B = Amount (nmole) of …

WebBuy Sodium monofluoroacetate, 99% (CAS No. 62-74-8) from Smolecule. Molecular Formula: C2H2FO2.Na C2H2FO2.Na CH2FCOONa C2H2FNaO2. Molecular Weight: 100.02 g/mol. Introduction Sodium monofluoroacetate, also known as 1080, is a highly toxic chemical compound that has been categorized as a Schedule 7 poison in Australia and … WebAug 24, 2024 · Determination of reactivation conditions for Synechocystis sp. PCC 6803 ... The mitochondrial aconitase activity from rat AF5 cells decreased to 48% and 19% of the control in the presence of 2 ...

Weblevels of aconitase, whereas its overexpression decreases its levels and activity [15, 25] (Fig. 1). This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. … Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic …

WebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by …

WebAug 1, 2005 · Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the ... bitsetter with vcarveWebDec 10, 2024 · Mitochondrial Aconitase In-Gel Enzyme Activity Assays Protein samples were separated using a non-denaturing gel, and then the gel was incubated in a coloring … dataplug by texaWebAug 31, 1999 · The aconitase protein of Bacillus subtilis was able to bind specifically to sequences resembling the iron response elements (IREs) found in eukaryotic mRNAs. The sequences bound include the rabbit ferritin IRE and IRE-like sequences in the B. subtilis operons that encode the major cytochrome oxidase and an iron uptake system. IRE … data plotting onlineWebOct 1, 1974 · TAA is also known to play a role in the TCA cycle where it inhibits the aconitase (ACO), the enzyme converting citrate to isocitrate via cis-aconitic acid (CAA) intermediate [34] [35] [36 ... bit setting c++data plus hospitality solutions reviewsWebApr 10, 2024 · This piece explains an activity that allows for the simple and accurate determination of the heat of vaporization, ΔH vap, of water at 100°C, and ultimately the approximate strength of a hydrogen bond in boiling water, in kJ·mol –1. The vaporization of water is an endothermic process represented by Equation 1: data plus in hueytownWebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent … data plus hospitality solutions