Epithelioma papulosum cyprini

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August undefined, 2024

WebIn this study, to know the role of HIF-1α in the apoptosis of fish cells, we produced HIF-1α knockout Epithelioma papulosum cyprini (EPC) cells using a CRISPR/Cas9 vector, and a single cell clone showing a heterozygous insertion/deletion (indel) mutation (one nucleotide insertion and one nucleotide deletion in HIF-1α gene) was chosen for further … WebAbstract. Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, …

A small RNA virus isolated from apparently healthy wild sandbar …

Web(i) Both epithelioma papulosum cyprini (EPC) and chinook salmon embryo (CHSE–214) cell lines must be maintained and used in all virus assays. Susceptible, normal appearing, and rapidly dividing cell cultures shall be selected. is sore breast a sign of breast cancer

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WebIHNV can be cultured using epithelioma papulosum cyprini (EPC) or fathead minnow cell lines, which required pretreatment of the homogenated tissue samples (e.g., spleen, … WebMar 14, 2024 · Epithelioma Papulosum Cyprini (EPC) cells were maintained at 24°C in GMEM/HEPES 25 mM medium supplemented with 2 mM L-glutamine (PAA) and with 10% fetal bovine serum (FBS) (Eurobio) . rVHSV were propagated in monolayer cultures of EPC cells at 15°C as previously described . Virus titers were determined by plaque assays on … WebTransmission electron microscopy of epithelioma papulosum cyprini cells infected with spring viremia of carp virus (SVCV): (a) cell nucleus (N; scale bar = 1 μ m), showing depolymerization and... i fight bears

Current lineages of the epithelioma papulosum cyprini …

Transmission electron microscopy of epithelioma papulosum cyprini …

WebMar 1, 2012 · Epithelioma papulosum cyprini (EPC) cells were cultured in Leibovitz medium (L-15, Sigma) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ml) and 10% fetal bovine serum (FBS, Gibco). The wild-type VHSV KJ2008 and the recombinant viruses rVHSV-wild and rVHSV-ΔNV-EGFP [18] were inoculated in … WebMar 22, 2024 · Furthermore, mutation of Leu27, Glu103, Lys117, and His165 markedly decreased the phosphorylation of signal transducer and activator of transcription (STAT) 1a induced by CiIFNa in the Epithelioma papulosum cyprini (EPC) cells, and Glu103 was shown to be required for the CiIFNa-activated antiviral activity. is sorel outlet for realWeb현재 Epithelioma Papulosum Cyprini (EPC) cell 배양중입니다. 계대배양해서 stock을 제... is sorelstores.com legit

"WebOct 28, 2024 · Epithelioma papulosum cyprini (EPC) cells were grown in Leibovitz medium (L-15, Sigma) enriched with 10% fetal bovine serum (FBS, Welgene) and penicillin-streptomycin (Welgene). Interferon regulatory factor 9 (IRF9) gene knockout EPC (ΔIRF9-EPC) cells established in our previous study [ 22] were also cultured under the same … " - Epithelioma papulosum cyprini

Epithelioma papulosum cyprini

Effect of miR-155 on type I interferon response in Epithelioma

Web西北农林科技大学水产科学系教授. 王高学，男，汉族，1965年9月生，陕西省富平人，西北农林科技大学水产科学系教授，博士，博士生导师，教育部水产类专业教学指导委员会委员；中国水产学会鱼病专业委员会委员；中国水产学会渔药专业委员会委员。. [1] WebEpithelioma papulosum cyprini cells were seeded at c. 90% confluency on a 48-well plate and incubated overnight in a 50-mg mL 1 IDU solution at 25 °C (Rovozzo & Burke 1973). Media was removed from the cell monolayers; the cells were then inoculated with tenfold dilutions of the virus sus-pension in IDU solution and incubated at room ...

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WebMar 1, 2001 · The variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) with the genes for -galactosidase from E.coli, for luciferases from... WebApr 13, 2024 · 线粒体提取试剂盒用于从动物细胞或组织中分离出完整而纯化的线粒体。. 适合于动物软组织（肝或脑组织）和硬组织（肌肉）以及培养细胞的线粒体的制备。. 其制备物产量高，可以被用于细胞凋亡、信号传递、代谢和蛋白组学等的研究。. 索莱宝的线粒体提取 ...

WebThe variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) with the genes for β-galactosidase from E. coli, for … WebJan 1, 2010 · Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, …

WebEPC [Epithelioma Papulosum Cyprini] is an epithelial cell line that was isolated in 1969 from the skin of a fathead minnow. This cell line was deposited by J Winton. Product category Animal cells Organism Pimephales promelas, Fathead minnow Cell type … WebMay 14, 2007 · Interestingly, the epithelioma papulosum Cyprini cells derived from Cyprinus carpio are restrictive to the virus. These results indicate that CyHV-3 is not restricted to common carp and koi, but other cyprinids are also vulnerable to the virus. Download to read the full article text References

Webepithelioma papulosum cyprini cells (EPC) and rainbow trout gonad cells (RTG-2) • Designed primers and cloned several genes from the IHNV genome into expression vectors such as pcDNA3.1(-) using ...

WebFeb 15, 2024 · To investigate the effect of CPF exposure on epithelioma papulosum cyprini (EPC) cell pyroptosis and the role of the miR-124-3p/CAPN1 axis, we … i fight dragons disaster hearts lyricsWebAbstract. MicroRNA-155 (miRNA-155) is known to play an important role in the regulation of innate and adaptive immune responses in mammals. However, no information is … i fight evilWebSep 17, 2024 · Citation 16 Moreover, in vitro study confirmed the role of chlorpyrifos in triggering epithelioma papulosum cyprini cell pyroptosis via miR-124-3p/CAPN1 axis. Citation 67. Conclusion. Similar to that in mammals, pyroptosis in fish is also activated by caspases through NLRP3 inflammasome to cleave GSDME and release its NT domain, … is sore mouth a zoonotic diseaseWebOct 1, 2004 · Several methods to improve transfection of epithelioma papulosum cyprini (EPC) carp cells have been tested and are reported here. By modifying the cell cycle state of EPC cell monolayers and ... i fight failWebMar 1, 2001 · Biology. Biotechnology Letters. The variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) … i fight depression every dayWebVolume 33, Issue 8p. 701-704 Current lineages of the epithelioma papulosum cyprini(EPC) cell line are contaminated with fathead minnow, Pimephales promelas, … is sore muscles good after exerciseWebApr 1, 2024 · LMBV demonstrates strong infectivity in epithelioma papulosum cyprini (EPC) cells and can cause the cytopathic effect (CPE) in this cell line. EPC cells were cultured in Medium 199/EBSS (M199, HyClone) supplemented with 10% fetal bovine serum (FBS, HyClone) containing streptomycin (100 μg/mL) and penicillin (100 μg/mL) at 25 °C. i fight for cancer signs