WebApr 1, 2024 · Targeted clearance of p16 Ink4-expressing cells in p16-INK-ATTAC (p16 Ink4a apoptosis through targeted activation of caspase) mice in the presence of AP20247 (a synthetic drug that dimerizes a membrane-bound myristoylated FK506-binding protein-caspase-8 [FKBP–Casp8] fusion protein) has been successfully used as a strategy to … WebApr 1, 2024 · The FKBP–Casp8 fusion protein is driven by a transcriptionally active element of the p16 promoter in SCs (Fig. 2A). New strategies targeting SCs. Above senolytic targets are usually shared by SCs and tumor cells, and almost all the targeted senolytics are repurposed anticancer agents. The repurposed senolytic agent usually have on-target …
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WebLed by veteran executives with hundreds of years of combined experience and with the financial backing of Columbia Capital, CPG is at the forefront of the data center services … WebApr 10, 2024 · Gene Name FKBP/Casp8; Type ORF; Subtype Suicide gene; Description FK506-binding protein 12 (with F36V mutation) fused with human caspase-8 protein … green foods organic beet juice powder
Naturally occurring p16Ink4a-positive cells shorten healthy …
The INK-ATTAC transgenic construct was made as follows. The FKBP–Casp8 fragment was subcloned from the aP2-ATTAC transgenic construct6 (a gift from P. Scherer) and inserted into pBlueScriptII (Stratagene). A 2,617-bp segment of the murine p16Ink4a promoter was PCR amplified from BAC DNA to replace … See more Prism software was used for the generation of all survival curves and statistical analyses. Two-tailed unpaired t tests were used for pairwise significance analysis in the … See more Culture of bone marrow cells was as previously described25. Briefly, tibia and femur bones of 2-month-old WT;INK-ATTAC transgenic mouse lines were collected and flushed with DMEM containing 15% FBS. … See more Bi-weekly checks for lordokyphosis and cataracts were performed as described15. Skeletal muscle fibre diameter measurements were performed on cross-sections of gastrocnemius and abdominal muscles of female … See more RNA extraction, cDNA synthesis and qRT–PCR from whole-mouse tissue were performed as previously described15. To perform qRT–PCR on GFP+ and GFP– cell populations of … See more WebJul 1, 2024 · Although CASP8 is typically activated by the death-induced signaling complex (DISC), a similar platform forms on autophagosomes and is required for complete enzyme activation . ATG12 can also promote apoptosis by binding to and inhibiting BCL2, a function required for full CYCS release during staurosporine-induced apoptosis ( 221 ). WebAP20247 (B/B Homodimerizer) is a chemical inducer of dimerization that activates FKBP-Casp8. Front Immunol, 2024, 13:1110322: S9726: Rimiducid (AP1903) Rimiducid (AP1903) is a chemical protein dimerizer and binds tightly to the target FKBP fusion protein while binding minimally to the abundant natural FKBP. Rimiducid elicites potent and dose ... flushing inboard outboard boat engines