High-speed counter-current chromatography
WebOct 7, 2024 · The high-speed counter-current chromatography (HSCCC) was implemented by employing a two-phase solvent system which consist of n-hexane–ethyl acetate–methanol-water (4:5:4:5, v/v/v/v) and n-hexane–ethyl acetate–methanol-water (1:5:1:5, v/v/v/v) to separate flavonoid into five fractions. WebOct 26, 2009 · The development of HPCCC instruments combined with the limitations that chemists are experiencing with solid/liquid chromatography techniques, has created an …
High-speed counter-current chromatography
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WebJournal of Chromatography B Volumes 1029–1030 , 1 September 2016 , Pages 191-197 Separation and purification of astaxanthin from Phaffia rhodozyma by preparative high-speed counter-current chromatography WebJul 26, 2012 · A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two …
WebAug 23, 2024 · This technique was named as High-Speed Countercurrent Chromatography or HSCCC, as the experimental duration was only 3 to 4 hours. Based on the principle of DCCC, Sanki (an instrument engineering ... WebFeb 12, 2024 · High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere Authors: Aneta Spórna-Kucab Cracow University of Technology...
WebIn this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum ( A. pendulum) Busch through an efficient high-speed counter-current chromatography （HSCCC）method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum … Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force and is used to separate, identify, and quantify the chemical components of a mixture. …
WebNov 22, 2024 · Finally, the targets were introduced to preparative high-speed counter-current chromatography for separation using ethyl acetate/n-butanol/acetic acid/water …
WebApr 21, 2016 · Counter current chromatography (CCC) is a liquid chromatography technique that uses two immiscible liquid phases and no solid support. 2. One liquid acts as the stationary phase and the other as the mobile phase. 3. In Dual Flow CCC/CPC both liquid phases are flowing, as would be common in counter current process extractors. 4. how to set up bell remoteWebHigh speed counter current chromatography (HSCCC) utilizes a liquid stationary-phase and, hence, does not suffer from irretrievable adsorption associated with conventional chromatography procedures. It is a good alternative for such techniques because of its … nothing about us without us quoteWebOct 26, 2013 · Abstract. This study presents an efficient strategy based on liquid–liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the … nothing about us without us tbsWebmachines were collectively known as high speed countercurrent chromatograms (hsccc). This breakthrough delivered two significant aspects of instrument performance: 1. Firstly, separation time was reduced by a factor of 10, so now these high capacity separations could be performed in times similar to solid phase chromatography, such as HPLC or ... how to set up bell streamerWebMay 23, 2008 · The effect of solvent system, partition coefficient, retention of stationary phase, column, revolution speed, and flow rate of mobile phase are well known parameters to effect HSCCC (high-speed counter-current chromatography) separations. Temperature effects on chromatographic techniques like HPLC a … how to set up bell answering machineWebHigh-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of squalene from microalgae. Crude squalene was … how to set up bellsouth.net email in outlookWebCountercurrent chromatography (CCC) belongs to the family of liquid partition chromatography but with one distinct feature: the system totally eliminates the use of a … how to set up bell satellite tv