Psectagb
WebThe variable region is indicated with a box. pSecTag B hCMV immediate early promoter Secretion signal Polylinker Primer binding sites C-terminal tag WebpSecTagB/sE2 and pSecTagB/sS1E2 containing the insert of E2-661 (aa384-661 of the HCV polyprotein) coding sequence downstream to a signal sequence of Igκ and under the control of the CMV promoter (Figure 1A) were used in the study.
Psectagb
Did you know?
WebFirst, a 2.9 kb compact plasmid vector (pVAC), containing CMV promoter, polycloning site, BGH poly (A) terminator, ampicillin resistance gene and pBR322 origin was constructed. A pVAC-hEPO was also constructed, which contained a human erythropoietin gene, for evaluating the transfection efficiency of naked plasmid DNA both in vitro and in vivo. WebSep 30, 2008 · MDA-MB-231 cells were transfected with pSecTagB-Slit2-HA and pSecTagB-Slit3-Myc and selected in zeocin (Invitrogen). n = 3 lines were generated expressing SLIT2-HA and n = 2 lines expressing SLIT3-Myc. Tumor generation. Stable cell lines (10 6 cells) were injected into precleared fat pads of nude mice.
WebAug 29, 2013 · The effects of pSecTagB-IFN-α on HBV mRNA, DNA and antigens were analyzed by real-time fluorescence quantitative PCR (qRT-PCR) and ELISA assays. RT … WebInvitrogen™ pSecTag2 A, B, & C Mammalian Expression Vectors. Designed for secretion, purification, and detection of fusion proteins. Supplier: Invitrogen™ V90020. Catalog No. …
Webmercial plasmid pSecTagB (Invitrogen) was utilized. The plasmid con-tains Ig k-chain leader sequence to ensure the secretion of expressed protein. cDNAs encoding selected … WebpSecTagB-IFN-α inhibits HBV replication Interferon remains a benchmark therapy for CHB [13]. We further investigated whether pSecTagB-IFN-α could play anti-HBV role by an …
WebMar 23, 2015 · The mammalian cell lines HEK293 and CHO have become important expression hosts in structural biology. Generating stable mammalian cell lines remains essential for studying the function and structure of recombinant proteins, despite the emergence of highly efficient transient transfection protocols. Production with stable cell …
Web3 Cloning into pSecTag2, continued Note If you wish to express and secrete your protein without the C-terminal tag, include a termination codon in your gene of interest. Note that … empire of the sun we rule the worldWebPlease login or register to sign up for Automatic Bill Pay. By connecting your checking or savings account to your PSEG account, your monthly energy bill will be paid automatically. … empire of the sun without makeupWebDNA断裂因子相似蛋白A抗体图片,我司提供的服务： （1）多种抗体纯化服务，如硫酸铵纯化、Protein A/G纯化、抗原亲和纯化等，帮助客户解决各种抗体纯化问题； （2）抗体标记服务，包括生物素标记、荧光素标记、酶标记等； （3）多种免疫检测分析 empire of the sun where to watchWebpSecTagB (Invitrogen, San Diego, CA). To generate the reelin–His domain expression vector (pSecF-reel-His), a pair of PCR primers, CGTCTAGACTTGGCAGTTCCGCAGGC (aa 206) and aa 27, were used. The PCR fragment was inserted into the HindIII and XbaI sites of pSecTagB (Invitrogen). Production of R2 and R5 antibodies. The pR2 and pR5 plasmids … empire of the sun without youWebDownload scientific diagram Lamivudine combined with pSecTagB-IFN-α displayed enhanced anti-HBV effect than with exogenously supplemented IFN-α. (A-C) HepG2.2.15 … empire of the sun where were his parentsWeb... pSecTagB/sE2 and pSecTagB/sS1E2 containing the insert of E2-661 (aa384-661 of the HCV polyprotein) coding sequence downstream to a signal sequence of Igκ and under the control of the CMV... empire of the sun wiz khalifaWebMay 4, 2007 · Exogenous Ang2 was encoded by a c-Myc and His-tagged pSecTagB expression vector that is described in the Supplementary Data and expressed in MCF-7 Ang2#1 cells. The associated Ang2 with β 1 integrin on various cells was assessed by immunoprecipitation separately with anti-β 1 ( A ), anti-c-Myc ( B ), or anti-Ang2 ( C ) … dr arash taghavi